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KMID : 1098420200280010029
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Korean Journal of Medicinal Crop Science
2020 Volume.28 No. 1 p.29 ~ p.37
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Deodorization of Gastrodia elata Blum Extracts using Diaion HP-20 Resin
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Jang Gwi-Yeong
Choi Je-Hun
Choi Su-Ji
Lee Seung-Eun
Seo Kyung-Hye
Kim Dong-Hwi
Jee Yun-Jeong
Jeong Heon-Sang
Lee Yoon-Jeong
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Abstract
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Background: Although Gastrodia elata contains the valuable, bio-active compounds gastrodin and 4-hydroxybenzyl alcohol (4-HBA), it also contain ρ-cresol, which is a major source of an objectionable pig slurry-like odor. Accordingly, this study was performed to determine the deodorization effect of Diaion HP-20 resin on a G. elata extract.
Methods and Results: Using Diaion HP-20 with an increasing concentration of ethanol, an extract of G. elata was fractionated into 15 fractions, which were anlayzed to determine their concentrations of active compounds and ρ-cresol. The gastrodin, 4-HBA, total phenolics, and ρ-cresol concentrations of the G. elata extract were 403.13, 408.15, 2,476.09, and 11.77 ㎍/㎖, respectively.
Among the different fractions, gastrodin and 4-HBA were detected in fractions 3 to- 6, and 4 to- 10, respectively whereas total phenolics were detected in all samples, with fractions 5 to- 7 being characterized by higher concentrations than were the other fractions. ρ-Cresol was detected in fractions 12 to- 15, at concentrations ranging from 0.39 ㎍/㎖ to 6.05 ㎍/㎖ with the highest concentration detected in fraction 14. The fractions containing ρ-cresol could be selectively separated from the G. elata extract without the loss of major bio-active compounds such as gastrodin and 4-HBA.
Conclusions: The results of this study indicate that a deodorizing method using Diaion HP-20 resin can be considered for improving the sensory qualities of G. elata extract without a loss of the major bio-active compounds.
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KEYWORD
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Gastrodia elata Blume, Deodorization, Diaion HP-20 Resin, Objectionable Odor
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